Novel genes in Human Asthma Based on a Mouse Model of Allergic Airway Inflammation and Human Investigations.

ABSTRACT:

Purpose

Based on a previous gene expression study in a mouse model of asthma, we selected 60 candidate genes and investigated their possible roles in human asthma.

Methods

In these candidate genes, 90 SNPs were genotyped using MassARRAY technology from 311 asthmatic children and 360 healthy controls of the Hungarian (Caucasian) population. Moreover, gene expression levels were measured by RT PCR in the induced sputum of 13 asthmatics and 10 control individuals. t-tests, chi-square tests, and logistic regression were carried out in order to assess associations of SNP frequency and expression level with asthma. Permutation tests were performed to account for multiple hypothesis testing.

Results

The frequency of 4 SNPs in 2 genes differed significantly between asthmatic

SUBMITTER: Temesi G 

PROVIDER: S-EPMC4214969 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature