From genes to protein mechanics on a chip.

ABSTRACT: Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.

SUBMITTER: Otten M 

PROVIDER: S-EPMC4216144 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature