High-throughput profiling identifies clinically actionable mutations in salivary duct carcinoma.

ABSTRACT:

Background

Salivary duct carcinoma (SDC) is a highly aggressive subtype of salivary gland cancers and there is no established standard therapy for this disease. Thus, development of molecular markers for SDC will be important to guide the diagnosis and therapy of this aggressive tumor.

Methods

We performed next-generation sequencing using the Ion Torrent AmpliSeq cancer panel, which explores the mutational status of hotspot regions in 50 cancer-associated genes, and we analyzed copy number variations (CNVs) of 21 genes by NanoString nCounter for 37 patients with SDC. Fluorescent in situ hybridization was also conducted to confirm ERBB2 gene amplification. Clinical records and tumor histopathology of the patients were retrospectively reviewed.

Results

Genetic alteratio

SUBMITTER: Ku BM 

PROVIDER: S-EPMC4216375 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature