Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo.

ABSTRACT: Mouse epiblast stem cells (EpiSCs) display temporal differences in the upregulation of Mixl1 expression during the initial steps of in vitro differentiation, which can be correlated with their propensity for endoderm differentiation. EpiSCs that upregulated Mixl1 rapidly during differentiation responded robustly to both Activin A and Nodal in generating foregut endoderm and precursors of pancreatic and hepatic tissues. By contrast, EpiSCs that delayed Mixl1 upregulation responded less effectively to Nodal and showed an overall suboptimal outcome of directed differentiation. The enhancement in endoderm potency in Mixl1-early cells may be accounted for by a rapid exit from the progenitor state and the efficient response to the induction of differentiation by Nodal. EpiSCs that readily differ

SUBMITTER: Kaufman-Francis K 

PROVIDER: S-EPMC4216471 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature