Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture.

ABSTRACT:

Summary

We isolate and characterize osteoblasts from humans without in vitro culture. These techniques should be broadly applicable to studying the pathogenesis of osteoporosis and other bone disorders.

Introduction

There is currently no data regarding the expression of specific genes or pathways in human osteoblasts that have not been subjected to extensive in vitro culture. Thus, we developed methods to rapidly isolate progressively enriched osteoblast populations from humans and characterized these cells.

Methods

Needle bone biopsies of the posterior iliac crest were subjected to sequential collagenase digests. The cells from the second digest were stained with an alkaline phosphatase (AP) antibody, and the AP+ cells were isolated using magnetic cell sorting.

Re

SUBMITTER: Fujita K 

PROVIDER: S-EPMC4216562 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature