The retinoblastoma tumor suppressor modulates DNA repair and radioresponsiveness.

ABSTRACT:

Purpose

Perturbations in the retinoblastoma pathway are over-represented in advanced prostate cancer; retinoblastoma loss promotes bypass of first-line hormone therapy. Conversely, preliminary studies suggested that retinoblastoma-deficient tumors may become sensitized to a subset of DNA-damaging agents. Here, the molecular and in vivo consequence of retinoblastoma status was analyzed in models of clinical relevance.

Experimental design

Experimental work was performed with multiple isogenic prostate cancer cell lines (hormone sensitive: LNCaP and LAPC4 cells and hormone resistant C42, 22Rv1 cells; stable knockdown of retinoblastoma using shRNA). Multiple mechanisms were interrogated including cell cycle, apoptosis, and DNA damage repair. Transcriptome analysis was performed,

SUBMITTER: Thangavel C 

PROVIDER: S-EPMC4216759 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature