Metabolic imaging: a link between lactate dehydrogenase A, lactate, and tumor phenotype.

ABSTRACT:

Purpose

We compared the metabolic profiles and the association between LDH-A expression and lactate production in two isogenic murine breast cancer cell lines and tumors (67NR and 4T1). These cell lines were derived from a single mammary tumor and have different growth and metabolic phenotypes.

Experimental design

LDH-A expression, lactate concentration, glucose utilization, and oxygen consumption were measured in cells, and the potential relationship between tumor lactate levels [measured by magnetic resonance spectroscopic imaging (MRSI)] and tumor glucose utilization [measured by [(18)F]2-deoxy-2-fluoro-D-glucose positron emission tomography ([(18)F]FDG-PET)] was assessed in orthotopic breast tumors derived from these cell lines.

Results

We show a substantial diffe

SUBMITTER: Serganova I 

PROVIDER: S-EPMC4217119 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature