Ontology highlight
ABSTRACT:
SUBMITTER: Hussain T
PROVIDER: S-EPMC4217140 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Cell 20141016 3
During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tR ...[more]