The γδ T-cell receptor repertoire is reconstituted in HIV patients after prolonged antiretroviral therapy.

ABSTRACT:

Objective

Determine whether reconstitution of Vγ2Vδ2 T cells in patients with HIV is due to new cell synthesis with recovery of the T-cell receptor repertoire or proliferative expansion of residual cells from the time of treatment initiation.

Design

Perform a cross-sectional analysis of the T-cell receptor complexity of Vγ2 chain in patients treated for HIV, natural virus suppressors who control viremia to undetectable levels, patients with chronic low-level viremia in the absence of therapy, and uninfected controls. Apply quantitative methods for repertoire analysis to assess the degree of Vδ2 repertoire loss or reconstitution.

Methods

T-cell receptor Vγ2 chain DNA clones (up to 300 per patient sample) were sequenced and aligned to enumerate the antigen-reactive subs

SUBMITTER: Chaudhry S 

PROVIDER: S-EPMC4217204 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature