Multicenter analytical evaluation of the automated electrochemiluminescence immunoassay for cyclosporine.

ABSTRACT:

Background

Cyclosporine A (CsA) is used as a posttransplantation immunosuppressant drug, and careful monitoring of CsA concentration in whole blood is essential. A new automated electrochemiluminescence immunoassay (ECLIA) for CsA measurement has been assessed in a multicenter evaluation.

Methods

Residual EDTA whole blood samples from patients undergoing CsA therapy after organ transplant were used in assay evaluation at 5 clinical laboratories in Europe. Experiments included imprecision according to CLSI EP5-A2 (within-run and intermediate), lower limit of quantification, linearity according to CLSI EP6-A, and recovery of commercial external quality control samples. In addition, comparisons to liquid chromatography-tandem mass spectrometry methods in routine use at each inv

SUBMITTER: Vogeser M 

PROVIDER: S-EPMC4218761 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature