LOH detected by microsatellite markers reveals the clonal origin of recurrent laryngeal squamous cell carcinoma.

ABSTRACT:

Background

The question of whether "recurrent" laryngeal carcinoma is truly a new tumour with a clonal origin that differs from that of the primary tumour has remained unanswered. The objective of this study was to determine whether recurrent tumours have the same genetic basis as primary tumours, as the answer to this question is important for the development of treatment strategies.

Materials and methods

Matched samples consisting of primary tumour, recurrent tumour and normal tissue were obtained from the same patient. A total of 37 patients with laryngeal cancer were examined for loss of heterozygosity (LOH) on the 3p, 5p, 7q, 8p, 9p, 13p, 17p and 18q chromosomal arms using PCR to amplify microsatellite markers. All patients were routinely followed up and 5-year survival

SUBMITTER: Cui Z 

PROVIDER: S-EPMC4218824 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature