In silico assay development for screening of tetracyclic triterpenoids as anticancer agents against human breast cancer cell line MCF7.

ABSTRACT: Experimental activity of a compound on cancer cell line/target is mostly analyzed in the form of percentage inhibition at different concentration gradient and time of incubation. In this study a statistical model has been developed referred as in silico assay using support vector regression model, which can act with change in concentration gradient and time of incubation. This model is a function of concentration gradient, treatment hour and independent components; which calculate the percentage inhibition in combination of above three components. This model is designed to screen tetracyclic triterpenoids active against human breast cancer cell line MCF7. The model has been statistically validated, checked for applicability domain and predicted results were reconfirmed by MTT assay, for ex

SUBMITTER: Prakash O 

PROVIDER: S-EPMC4218838 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature