High-throughput sequencing of human immunoglobulin variable regions with subtype identification.

ABSTRACT: The humoral immune response plays a critical role in controlling infection, and the rapid adaptation to a broad range of pathogens depends on a highly diverse antibody repertoire. The advent of high-throughput sequencing technologies in the past decade has enabled insights into this immense diversity. However, not only the variable, but also the constant region of antibodies determines their in vivo activity. Antibody isotypes differ in effector functions and are thought to play a defining role in elicitation of immune responses, both in natural infection and in vaccination. We have developed an Illumina MiSeq high-throughput sequencing protocol that allows determination of the human IgG subtype alongside sequencing full-length antibody variable heavy chain regions. We thereby took advanta

SUBMITTER: Schanz M 

PROVIDER: S-EPMC4218849 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature