MicroRNA regulation in human CD8+ T cell subsets--cytokine exposure alone drives miR-146a expression.

ABSTRACT:

Background

microRNAs (miRNAs) are emerging as key regulators of the immune system, but their role in CD8+ T cell differentiation is not well explored. Some evidence suggests that signals from cell surface receptors influence the expression of miRNAs in CD8+ T cells, and may have consequent effects on cell phenotype and function. We set out to investigate whether common gamma chain cytokines modulated human CD8+ T cell expression of miR-146a, which previous studies have associated with different stages of CD8+ differentiation. We also investigated how changes in miR-146a related to other miRNAs that alter with CD8+ differentiation status.

Methods

We treated human CD8+ T cells with the cytokines IL-2, IL-7 or IL-15 either at rest or after stimulation with anti-CD3 and anti-CD2

SUBMITTER: Sheppard HM 

PROVIDER: S-EPMC4219087 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature