Genome-wide profiling of mouse RNA secondary structures reveals key features of the mammalian transcriptome.

ABSTRACT:

Background

The understanding of RNA structure is a key feature toward the comprehension of RNA functions and mechanisms of action. In particular, non-coding RNAs are thought to exert their functions by specific secondary structures, but an efficient annotation on a large scale of these structures is still missing.

Results

By using a novel high-throughput method, named chemical inference of RNA structures, CIRS-seq, that uses dimethyl sulfate, and N-cyclohexyl- N'-(2-morpholinoethyl) carbodiimide metho-p-toluenesulfonate to modify RNA residues in single-stranded conformation within native deproteinized RNA secondary structures, we investigate the structural features of mouse embryonic stem cell transcripts. Our analysis reveals an unexpected higher structuring of the 5′ and 3

SUBMITTER: Incarnato D 

PROVIDER: S-EPMC4220049 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature