Pyrroloquinoline quinone protects mouse brain endothelial cells from high glucose-induced damage in vitro.

ABSTRACT:

Aim

To investigate the effects of pyrroloquinoline quinone (PQQ), an oxidoreductase cofactor, on high glucose-induced mouse endothelial cell damage in vitro.

Methods

Mouse brain microvascular endothelial bEND.3 cells were exposed to different glucose concentrations (5.56, 25 and 40 mmol/L) for 24 or 48 h. The cell viability was examined using MTT assay. Flow cytometry was used to analyze the apoptosis and ROS levels in the cells. MitoTracker Green staining was used to examine the mitochondria numbers in the cells. Western blot analysis was used to analyze the expression of HIF-1α and the proteins in JNK pathway.

Results

Treatment of bEND.3 cells with high glucose significantly decreased the cell viability, while addition of PQQ (1 and 10 μmol/L) reversed the high gluc

SUBMITTER: Wang Z 

PROVIDER: S-EPMC4220070 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature