Identification of novel genes conferring altered azole susceptibility in Aspergillus fumigatus.

ABSTRACT: Azoles are currently the mainstay of antifungal treatment both in agricultural and in clinical settings. Although the target site of azole action is well studied, the basis of azole resistance and the ultimate mode of action of the drug in fungi are poorly understood. To gain a deeper insight into these aspects of azole action, restriction-mediated plasmid integration (REMI) was used to create azole sensitive and resistant strains of the clinically important fungus Aspergillus fumigatus. Four azole sensitive insertions and four azole-resistant insertions were characterized. Three phenotypes could be re-created in wild-type AF210 by reintegration of rescued plasmid and a further four could be confirmed by complementation of the mutant phenotype with a copy of the wild-type gene predicted to

SUBMITTER: Bowyer P 

PROVIDER: S-EPMC4220099 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature