¹H NMR Metabolomics Study of Metastatic Melanoma in C57BL/6J Mouse Spleen.

ABSTRACT: Melanoma is a malignant tumor of melanocytes. Although extensive investigations have been done to study metabolic changes in primary melanoma in vivo and in vitro, little effort has been devoted to metabolic profiling of metastatic tumors in organs other than lymph nodes. In this work, NMR-based metabolomics combined with multivariate data analysis is used to study metastatic B16-F10 melanoma in C57BL/6J mouse spleen. Principal Component Analysis (PCA), an unsupervised multivariate data analysis method, is used to detect possible outliers, while Orthogonal Projection to Latent Structure (OPLS), a supervised multivariate data analysis method, is employed to find important metabolites responsible for discriminating the control and the melanoma groups. Two different strategies, i.e. spectral

SUBMITTER: Wang X 

PROVIDER: S-EPMC4220309 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature