Localization of the CyanoP binding site on photosystem II by surface plasmon resonance spectroscopy.

ABSTRACT: Photosystem II (PSII), a large multi subunit membrane protein complex localized in the thylakoid membrane of cyanobacteria and chloroplasts, is the only known enzyme that catalyzes the light-driven oxidation of water. In addition to the membrane intrinsic part of PSII, efficient oxygen evolution requires soluble protein subunits at its luminal interface. In contrast to the detailed crystal structure of the active cyanobacterial complex the characterization of intermediate PSII species related to its assembly and repair is hampered by their instability or low abundance. As most structural variations of the corresponding PSII species are based on a different set of protein factors bound to the luminal interface of the complex we developed a system for interaction analysis between PSII and it

SUBMITTER: Cormann KU 

PROVIDER: S-EPMC4220643 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature