Loss of LKB1 in high-grade endometrial carcinoma: LKB1 is a novel transcriptional target of p53.

ABSTRACT:

Background

Liver kinase B1 (LKB1) is a serine/threonine kinase that functions as a tumor suppressor and regulates cell polarity, proliferation, and metabolism. Mutations in LKB1 are associated with Peutz-Jeghers syndrome as well as sporadic cervical and lung cancers. Although LKB1-null mice develop invasive endometrial cancers, the role and regulation of LKB1 in the pathogenesis of human endometrial cancer are not well defined and are the focus of these studies.

Methods

LKB1 protein and messenger RNA (mRNA) expression levels were evaluated in high-grade and low-grade endometrioid endometrial cancer (EEC) and cell lines by reverse transcriptase-polymerase chain reaction analysis, Western blot analysis, and immunohistochemistry. Mutational and promoter analyses of the LKB1 gen

SUBMITTER: Co NN 

PROVIDER: S-EPMC4221493 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature