The application of Gaussian mixture models for signal quantification in MALDI-TOF mass spectrometry of peptides.

ABSTRACT: Matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) coupled with stable isotope standards (SIS) has been used to quantify native peptides. This peptide quantification by MALDI-TOF approach has difficulties quantifying samples containing peptides with ion currents in overlapping spectra. In these overlapping spectra the currents sum together, which modify the peak heights and make normal SIS estimation problematic. An approach using Gaussian mixtures based on known physical constants to model the isotopic cluster of a known compound is proposed here. The characteristics of this approach are examined for single and overlapping compounds. The approach is compared to two commonly used SIS quantification methods for single compound, namely Peak Intensity method and Riemann su

SUBMITTER: Spainhour JC 

PROVIDER: S-EPMC4221630 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature