The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.

ABSTRACT:

Background

The S-phase checkpoint aims to prevent cells from generation of extensive single-stranded DNA that predisposes to genome instability. The S. cerevisiae complex Tof1/Csm3/Mrc1 acts to restrain the replicative MCM helicase when DNA synthesis is prohibited. Keeping the replication machinery intact allows restart of the replication fork when the block is relieved. Although the subunits of the Tof1/Csm3/Mrc1 complex are well studied, the impact of every single subunit on the triple complex formation and function needs to be established.

Findings

This work studies the cellular localization and the chromatin binding of GFP-tagged subunits when the complex is intact and when a subunit is missing. We demonstrate that the complex is formed in cell nucleus, not the cytoplasm

SUBMITTER: Uzunova SD 

PROVIDER: S-EPMC4221646 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature