Dynamic F-actin movement is essential for fertilization in Arabidopsis thaliana.

ABSTRACT: In animals, microtubules and centrosomes direct the migration of gamete pronuclei for fertilization. By contrast, flowering plants have lost essential components of the centrosome, raising the question of how flowering plants control gamete nuclei migration during fertilization. Here, we use Arabidopsis thaliana to document a novel mechanism that regulates F-actin dynamics in the female gametes and is essential for fertilization. Live imaging shows that F-actin structures assist the male nucleus during its migration towards the female nucleus. We identify a female gamete-specific Rho-GTPase that regulates F-actin dynamics and further show that actin-myosin interactions are also involved in male gamete nucleus migration. Genetic analyses and imaging indicate that microtubules are dispensabl

SUBMITTER: Kawashima T 

PROVIDER: S-EPMC4221737 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature