A systems biology analysis of the changes in gene expression via silencing of HPV-18 E1 expression in HeLa cells.

ABSTRACT: Previous studies have reported the detection of a truncated E1 mRNA generated from HPV-18 in HeLa cells. Although it is unclear whether a truncated E1 protein could function as a replicative helicase for viral replication, it would still retain binding sites for potential interactions with different host cell proteins. Furthermore, in this study, we found evidence in support of expression of full-length HPV-18 E1 mRNA in HeLa cells. To determine whether interactions between E1 and cellular proteins play an important role in cellular processes other than viral replication, genome-wide expression profiles of HPV-18 positive HeLa cells were compared before and after the siRNA knockdown of E1 expression. Differential expression and gene set enrichment analysis uncovered four functionally relat

SUBMITTER: Castillo A 

PROVIDER: S-EPMC4221889 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature