Improving microbial biogasoline production in Escherichia coli using tolerance engineering.

ABSTRACT:

Unlabelled

Engineering microbial hosts for the production of fungible fuels requires mitigation of limitations posed on the production capacity. One such limitation arises from the inherent toxicity of solvent-like biofuel compounds to production strains, such as Escherichia coli. Here we show the importance of host engineering for the production of short-chain alcohols by studying the overexpression of genes upregulated in response to exogenous isopentenol. Using systems biology data, we selected 40 genes that were upregulated following isopentenol exposure and subsequently overexpressed them in E. coli. Overexpression of several of these candidates improved tolerance to exogenously added isopentenol. Genes conferring isopentenol tolerance phenotypes belonged to diverse functional

SUBMITTER: Foo JL 

PROVIDER: S-EPMC4222104 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature