Subventricular zone neuronal progenitors undergo multiple divisions and retract their processes prior to each cytokinesis.

ABSTRACT: Mitotically active progenitor cells from the anterior portion of the forebrain subventricular zone (SVZa), which give rise throughout life to olfactory bulb interneurons, bear processes and express neuronal markers. To understand how rodent SVZa neuronal progenitors coordinate division and process formation, we used time-lapse videomicroscopy to analyse the proliferative behavior of SVZa progenitors in dissociated cell culture continuously for up to five generations. The cell cycle time of these cultured SVZa cells assessed videomicroscopically (cytokinesis to cytokinesis) was similar to the cell cycle time along the rostral migratory stream in vivo (14-17 h). The relationship between process extension, process retraction and cytokinesis was assessed quantitatively for 120 cells undergoing

SUBMITTER: Coskun V 

PROVIDER: S-EPMC4222250 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature