Identification of cardiac myofilament protein isoforms using multiple mass spectrometry based approaches.

ABSTRACT:

Purpose

The identification of protein isoforms in complex biological samples is challenging. We, therefore, used an MS approach to unambiguously identify cardiac myofilament protein isoforms based on the observation of a tryptic peptide consisting of a sequence unique to a particular isoform.

Experimental design

Three different workflows were used to isolate and fractionate rat cardiac myofilament subproteomes. All fractions were analyzed on an LTQ-Orbitrap MS, proteins were identified using various search engines (MASCOT, X!Tandem, X!Tandem Kscore, and OMSSA) with results combined via PepArML Meta-Search engine, and a postsearch analysis was performed by MASPECTRAS. All MS data have been deposited in the ProteomeXchange with identifier PXD000874 (http://proteomecentral.prot

SUBMITTER: Kooij V 

PROVIDER: S-EPMC4222538 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature