Dielectrophoretic monitoring and interstrain separation of intact Clostridium difficile based on their S(Surface)-layers.

ABSTRACT: Clostridium difficile (C. difficile) infection (CDI) rates have exhibited a steady rise worldwide over the last two decades and the infection poses a global threat due to the emergence of antibiotic resistant strains. Interstrain antagonistic interactions across the host microbiome form an important strategy for controlling the emergence of CDI. The current diagnosis method for CDI, based on immunoassays for toxins produced by pathogenic C. difficile strains, is limited by false negatives due to rapid toxin degradation. Furthermore, simultaneous monitoring of nontoxigenic C. difficile strains is not possible, due to absence of these toxins, thereby limiting its application toward the control of CDI through optimizing antagonistic interstrain interactions. Herein, we demonstrate that morpho

SUBMITTER: Su YH 

PROVIDER: S-EPMC4222627 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature