MiR-181a contributes to bufalin-induced apoptosis in PC-3 prostate cancer cells.

ABSTRACT:

Background

Bufalin is a major active compound of cinobufacini, which comes from dried toad venom and has been used for treatments of various cancers in China for many years. A number of studies have demonstrated that bufalin can induce apoptosis in some cancers. However, effects and mechanism of bufalin on prostate cancer cells remain unknown.

Methods

Apoptosis assay was measured by the annexin-V/PI flow cytometric assay. Western blot was used to measure Caspase-3 and Bcl-2. qRT-PCR was used to measure the relative expression of miR-181a.

Results

Bufalin was found to induce the expression of miR-181a, a small non-coding RNA believed to induce apoptosis by repressing its target gene, BCL-2. In prostate cancer PC-3cell line, bufalin-induced apoptosis can be largely atte

SUBMITTER: Zhai XF 

PROVIDER: S-EPMC4222721 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature