ARTIST: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing.

ABSTRACT: Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic requirements for bacterial growth in different conditions, as it enables simultaneous genome-wide analysis of the fitness of thousands of mutants. However, current methods for comparative analysis of TIS data do not adjust for stochastic experimental variation between datasets and are limited to interrogation of annotated genomic elements. Here, we present ARTIST, an accessible TIS analysis pipeline for identifying essential regions that are required for growth under optimal conditions as well as conditionally essential loci that participate in survival only under specific conditions. ARTIST uses simulation-based normalization to model and compensate for experimental noise, and thereby enhances the statist

SUBMITTER: Pritchard JR 

PROVIDER: S-EPMC4222735 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature