Predictive gene signature of response to the anti-TweakR mAb PDL192 in patient-derived breast cancer xenografts.

ABSTRACT:

Purpose

(1) To determine TweakR expression in human breast cancers (BC), (2) evaluate the antitumor effect of the anti-TweakR antibody PDL192, used alone or after chemotherapy-induced complete remission (CR), on patient-derived BC xenografts (PDX) and (3) define predictive markers of response.

Experimental design

TweakR expression was analyzed by IHC on patients and PDXs BC samples. In vivo antitumor effect of PDL192 was evaluated on eight TweakR-positive BC PDXs alone or after complete remission induced by a combination of doxorubicin and cyclophosphamide. Using both responding and resistant PDX tumors after PDL192 administration, RT-QPCR were performed on a wide list of selected candidate genes to identify predictive markers of response.

Results

TweakR protein was e

SUBMITTER: de Plater L 

PROVIDER: S-EPMC4222831 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature