Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer.

ABSTRACT:

Background and objectives

Transcription factor 3 (TCF3) implicates Wnt signaling pathway and regulates E-cadherin expression, which is involved in aggressiveness of tumors. This study aims to investigate the role of TCF3 in predicting prognosis of patients with stage II and III colorectal cancer (CRC).

Methods

Real-Time quantitative PCR was performed in 64 fresh CRC tissues and 6 cell lines to examine TCF3 mRNA expression. TCF3 protein expression dynamics were detected by immunohistochemistry of 118 paraffin-embedded specimens, and the clinical significance of TCF3 was assessed by clinical correlation and Kaplan-Meier analyses. Aberrant hypomethylation of TCF3 promoter was also investigated using bisulfite sequencing and methylation specific PCR.

Results

The up-regula

SUBMITTER: Li C 

PROVIDER: S-EPMC4222969 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature