Differential effects of tissue culture coating substrates on prostate cancer cell adherence, morphology and behavior.

ABSTRACT: Weak cell-surface adhesion of cell lines to tissue culture surfaces is a common problem and presents technical limitations to the design of experiments. To overcome this problem, various surface coating protocols have been developed. However, a comparative and precise real-time measurement of their impact on cell behavior has not been conducted. The prostate cancer cell line LNCaP, derived from a patient lymph node metastasis, is a commonly used model system in prostate cancer research. However, the cells' characteristically weak attachment to the surface of tissue culture vessels and cover slips has impeded their manipulation and analysis and use in high throughput screening. To improve the adherence of LNCaP cells to the culture surface, we compared different coating reagents (poly-L-lys

SUBMITTER: Liberio MS 

PROVIDER: S-EPMC4223027 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature