Proton transfer in the K-channel analog of B-type Cytochrome c oxidase from Thermus thermophilus.

ABSTRACT: A key enzyme in aerobic metabolism is cytochrome c oxidase (CcO), which catalyzes the reduction of molecular oxygen to water in the mitochondrial and bacterial membranes. Substrate electrons and protons are taken up from different sides of the membrane and protons are pumped across the membrane, thereby generating an electrochemical gradient. The well-studied A-type CcO uses two different entry channels for protons: the D-channel for all pumped and two consumed protons, and the K-channel for the other two consumed protons. In contrast, the B-type CcO uses only a single proton input channel for all consumed and pumped protons. It has the same location as the A-type K-channel (and thus is named the K-channel analog) without sharing any significant sequence homology. In this study, we perform

SUBMITTER: Woelke AL 

PROVIDER: S-EPMC4223178 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature