Membrane-induced structural rearrangement and identification of a novel membrane anchor in talin F2F3.

ABSTRACT: Experimental challenges associated with characterization of the membrane-bound form of talin have prevented us from understanding the molecular mechanism of its membrane-dependent integrin activation. Here, utilizing what we believe to be a novel membrane mimetic model, we present a reproducible model of membrane-bound talin observed across multiple independent simulations. We characterize both local and global membrane-induced structural transitions that successfully reconcile discrepancies between biochemical and structural studies and provide insight into how talin might modulate integrin function. Membrane binding of talin, captured in unbiased simulations, proceeds through three distinct steps: initial electrostatic recruitment of the F2 subdomain to anionic lipids via several basic r

SUBMITTER: Arcario MJ 

PROVIDER: S-EPMC4223205 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature