A critical residue selectively recruits nucleotides for t7 RNA polymerase transcription fidelity control.

ABSTRACT: Nucleotide selection is essential for fidelity control in gene replication and transcription. Recent work on T7 RNA polymerase suggested that a small posttranslocation free energy bias stabilizes Tyr(639) in the active site to aid nucleotide selection. However, it was not clear exactly how Tyr(639) assists the selection. Here we report a molecular-dynamics simulation study revealing atomistic detail of this critical selectivity. The study shows first that Tyr(639) blocks the active site at posttranslocation by marginally stacking to the end basepair of the DNA-RNA hybrid. The study then demonstrates that at the nucleotide preinsertion state, a cognate RNA nucleotide does not affect the local Tyr(639) stabilization, whereas a noncognate nucleotide substantially stabilizes Tyr(639) so that T

SUBMITTER: Duan B 

PROVIDER: S-EPMC4223216 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature