Structural characterization by multistage mass spectrometry (MSn) of human milk glycans recognized by human rotaviruses.

ABSTRACT: We have shown that recombinant forms of VP8* domains of the human rotavirus outer capsid spike protein VP4 from human neonatal strains (N155(G10P[11]) and RV3(G3P[6]) and a bovine strain (B223) recognize unique glycans within the repertoire of human milk glycans. The accompanying study by Yu et al.(2), describes a human milk glycan shotgun glycan microarray that led to the identification of 32 specific glycans in the human milk tagged glycan library that were recognized by these human rotaviruses. These microarray analyses also provided a variety of metadata about the recognized glycan structures compiled from anti-glycan antibody and lectin binding before and after specific glycosidase digestions, along with compositional information from mass analysis by matrix-assisted laser desorption

SUBMITTER: Ashline DJ 

PROVIDER: S-EPMC4223484 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature