Quantitative mass spectrometry reveals plasticity of metabolic networks in Mycobacterium smegmatis.

ABSTRACT: Mycobacterium tuberculosis has a remarkable ability to persist within the human host as a clinically inapparent or chronically active infection. Fatty acids are thought to be an important carbon source used by the bacteria during long term infection. Catabolism of fatty acids requires reprogramming of metabolic networks, and enzymes central to this reprogramming have been targeted for drug discovery. Mycobacterium smegmatis, a nonpathogenic relative of M. tuberculosis, is often used as a model system because of the similarity of basic cellular processes in these two species. Here, we take a quantitative proteomics-based approach to achieve a global view of how the M. smegmatis metabolic network adjusts to utilization of fatty acids as a carbon source. Two-dimensional liquid chromatography

SUBMITTER: Chopra T 

PROVIDER: S-EPMC4223488 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature