A systematic proteomic analysis of Listeria monocytogenes house-keeping protein secretion systems.

ABSTRACT: Listeria monocytogenes is a firmicute bacterium causing serious infections in humans upon consumption of contaminated food. Most of its virulence factors are secretory proteins either released to the medium or attached to the bacterial surface. L. monocytogenes encodes at least six different protein secretion pathways. Although great efforts have been made in the past to predict secretory proteins and their secretion routes using bioinformatics, experimental evidence is lacking for most secretion systems. Therefore, we constructed mutants in the main housekeeping protein secretion systems, which are the Sec-dependent transport, the YidC membrane insertases SpoIIIJ and YqjG, as well as the twin-arginine pathway, and analyzed their secretion and virulence defects. Our results demonstrate tha

SUBMITTER: Halbedel S 

PROVIDER: S-EPMC4223492 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature