A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients.

ABSTRACT:

Background

Nosocomial candidaemia is associated with high mortality rates in critically ill paediatric patients; thus, the early detection and identification of the infectious agent is crucial for successful medical intervention. The PCR-based techniques have significantly increased the detection of Candida species in bloodstream infections. In this study, a multiplex nested PCR approach was developed for candidaemia detection in neonatal and paediatric intensive care patients.

Methods

DNA samples from the blood of 54 neonates and children hospitalised in intensive care units with suspected candidaemia were evaluated by multiplex nested PCR with specific primers designed to identify seven Candida species, and the results were compared with those obtained from blood cultures.

SUBMITTER: Taira CL 

PROVIDER: S-EPMC4223582 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature