Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum.

ABSTRACT: Although microbes directly accepting electrons from a cathode have been applied for CO2 reduction to produce multicarbon-compounds, a high electron demand and low product concentration are critical limitations. Alternatively, the utilization of electrons as a co-reducing power during fermentation has been attempted, but there must be exogenous mediators due to the lack of an electroactive heterotroph. Here, we show that Clostridium pasteurianum DSM 525 simultaneously utilizes both cathode and substrate as electron donors through direct electron transfer. In a cathode compartment poised at +0.045 V vs. SHE, a metabolic shift in C. pasteurianum occurs toward NADH-consuming metabolite production such as butanol from glucose (20% shift in terms of NADH consumption) and 1,3-propandiol from glyc

SUBMITTER: Choi O 

PROVIDER: S-EPMC4223642 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature