Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates.

ABSTRACT: Cells sense and interpret mechanical cues, including cell-cell and cell-substrate interactions, in the microenvironment to collectively regulate various physiological functions. Understanding the influences of these mechanical factors on cell behavior is critical for fundamental cell biology and for the development of novel strategies in regenerative medicine. Here, we demonstrate plasma lithography patterning on elastomeric substrates for elucidating the influences of mechanical cues on neuronal differentiation and neuritogenesis. The neuroblastoma cells form neuronal spheres on plasma-treated regions, which geometrically confine the cells over two weeks. The elastic modulus of the elastomer is controlled simultaneously by the crosslinker concentration. The cell-substrate mechanical inter

SUBMITTER: Nam KH 

PROVIDER: S-EPMC4223667 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature