Hypoxia represses microRNA biogenesis proteins in breast cancer cells.

ABSTRACT:

Background

Cancers are commonly characterised by hypoxia and also by global reductions in the levels of mature microRNAs. We have examined the hypothesis that hypoxia might mediate this reduction through repressive effects on microRNA biogenesis proteins.

Methods

Breast cancer cell lines were exposed to hypoxia and manipulations of hypoxia inducible factor (HIF) and HIF hydroxylase activity. The effects of hypoxia on the mRNA and protein levels of enzymes involved in microRNA biogenesis (Dicer, Drosha, TARPB2, DCGR8, XPO5) was determined by RT PCR and immunoblotting. The effect of hypoxia on microRNAs was determined with microarray studies, RT PCR and reporter assays.

Results

In breast cancer lines there was significant reduction of Dicer mRNA and protein levels in ce

SUBMITTER: Bandara V 

PROVIDER: S-EPMC4223767 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature