Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity.

ABSTRACT:

Background

Allergy diagnosis by determination of allergen-specific IgE is complicated by clinically irrelevant IgE, of which the most prominent example is IgE against cross-reactive carbohydrate determinants (CCDs) that occur on allergens from plants and insects. Therefore, CCDs cause numerous false-positive results. Inhibition of CCDs has been proposed as a remedy, but has not yet found its way into the routine diagnostic laboratory. We sought to provide a simple and affordable procedure to overcome the CCD problem.

Methods

Serum samples from allergic patients were analysed for allergen-specific IgEs by different commercial tests (from Mediwiss, Phadia and Siemens) with and without a semisynthetic CCD blocker with minimized potential for nonspecific interactions that was pr

SUBMITTER: Holzweber F 

PROVIDER: S-EPMC4223978 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature