High-resolution specificity from DNA sequencing highlights alternative modes of Lac repressor binding.

ABSTRACT: Knowing the specificity of transcription factors is critical to understanding regulatory networks in cells. The lac repressor-operator system has been studied for many years, but not with high-throughput methods capable of determining specificity comprehensively. Details of its binding interaction and its selection of an asymmetric binding site have been controversial. We employed a new method to accurately determine relative binding affinities to thousands of sequences simultaneously, requiring only sequencing of bound and unbound fractions. An analysis of 2560 different DNA sequence variants, including both base changes and variations in operator length, provides a detailed view of lac repressor sequence specificity. We find that the protein can bind with nearly equal affinities to opera

SUBMITTER: Zuo Z 

PROVIDER: S-EPMC4224169 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature