Genome resilience and prevalence of segmental duplications following fast neutron irradiation of soybean.

ABSTRACT: Fast neutron radiation has been used as a mutagen to develop extensive mutant collections. However, the genome-wide structural consequences of fast neutron radiation are not well understood. Here, we examine the genome-wide structural variants observed among 264 soybean [Glycine max (L.) Merrill] plants sampled from a large fast neutron-mutagenized population. While deletion rates were similar to previous reports, surprisingly high rates of segmental duplication were also found throughout the genome. Duplication coverage extended across entire chromosomes and often prevailed at chromosome ends. High-throughput resequencing analysis of selected mutants resolved specific chromosomal events, including the rearrangement junctions for a large deletion, a tandem duplication, and a translocation.

SUBMITTER: Bolon YT 

PROVIDER: S-EPMC4224183 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature