Well Plate Circular Dichroism Reader for the Rapid Determination of Enantiomeric Excess.

ABSTRACT: Circular dichroism (CD) spectropolarimeters typically employ one photoelastic modulator. However, spectropolarimeters employing two or even four modulators are more versatile and can be used to subvert common measurement errors arising from imperfectly isotropic samples or sample holders. Small linear anisotropies that can cause large errors in CD measurement can be associated with multi-well sample holders. Thus, high-throughput CD analyses in multi-well plates have not yet been demonstrated. One such application is the determination of enantiomeric excess of a library of reaction products. Herein, a spectropolarimeter employing four photoelastic modulators and a translation stage was used to determine the enantiomeric excess of a family of chiral amine complexes much

SUBMITTER: Metola P 

PROVIDER: S-EPMC4224325 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature