Automated nucleic acid chain tracing in real time.

ABSTRACT: The crystallographic structure solution of nucleotides and nucleotide complexes is now commonplace. The resulting electron-density maps are often poorer than for proteins, and as a result interpretation in terms of an atomic model can require significant effort, particularly in the case of large structures. While model building can be performed automatically, as with proteins, the process is time-consuming, taking minutes to days depending on the software and the size of the structure. A method is presented for the automatic building of nucleotide chains into electron density which is fast enough to be used in interactive model-building software, with extended chain fragments built around the current view position in a fraction of a second. The speed of the method arises from the determina

SUBMITTER: Cowtan K 

PROVIDER: S-EPMC4224457 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature