Fast photochemical oxidation of proteins (FPOP) maps the epitope of EGFR binding to adnectin.

ABSTRACT: Epitope mapping is an important tool for the development of monoclonal antibodies, mAbs, as therapeutic drugs. Recently, a class of therapeutic mAb alternatives, adnectins, has been developed as targeted biologics. They are derived from the 10th type III domain of human fibronectin ((10)Fn3). A common approach to map the epitope binding of these therapeutic proteins to their binding partners is X-ray crystallography. Although the crystal structure is known for Adnectin 1 binding to human epidermal growth factor receptor (EGFR), we seek to determine complementary binding in solution and to test the efficacy of footprinting for this purpose. As a relatively new tool in structural biology and complementary to X-ray crystallography, protein footprinting coupled with mass spectrometry is promis

SUBMITTER: Yan Y 

PROVIDER: S-EPMC4224620 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature