A mouse protein that localizes to acrosome and sperm tail is regulated by Y-chromosome.

ABSTRACT:

Background

Acrosomal proteins play crucial roles in the physiology of fertilization. Identification of proteins localizing to the acrosome is fundamental to the understanding of its contribution to fertilization. Novel proteins are still being reported from acrosome. In order to capture yet unreported proteins localizing to acrosome in particular and sperm in general, 2D-PAGE and mass spectrometry analysis of mouse sperm proteins was done.

Results

One of the protein spots identified in the above study was reported in the NCBI database as a hypothetical protein from Riken cDNA 1700026L06 that localizes to chromosome number 2. Immunofluorescence studies using the antibody raised in rabbit against the recombinant protein showed that it localized to mouse acrosome and sperm tail

SUBMITTER: Bhattacharya R 

PROVIDER: S-EPMC4225516 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature